Short reports
ScienceAsia 37 (2011): 373-376 |doi:
10.2306/scienceasia1513-1874.2011.37.373
Regeneration of lily (Lilium longiflorum `Easter lily') by callus derived from leaf explants cultured in vitro
Kantamaht Kanchanapooma, Tidarat Ponpiboona, Wimon Wirakiatb, Kamnoon Kanchanapoomb,*
ABSTRACT: Bulb scales of Lilium longiflorum `Easter lily' were cultured in vitro on Murashige and Skoog (MS) medium containing various combinations and concentrations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA). The MS medium supplemented with 15 µM BA and 0.5 µM NAA produced the largest numbers of multiple shoots (100%) after one month of culture. The MS medium containing 5 µM indole-3-butyric acid was effective for induction of roots. When individual isolated leaves from regenerated shoots were transferred to MS medium supplemented with 2,4-dichlorophenoxyacetate (2,4-D) and NAA, a yellow compact callus was obtained. The calluses differentiated into adventitious shoots and a high frequency of shoot organogenesis (70%) was found on MS medium containing 18 µM 2,4-D with 14 shoots.
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Centre for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand |
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Plant Biotechnology Research Unit, Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand |
* Corresponding author, E-mail: kantamaht@hotmail.com
Received 4 May 2011, Accepted 2 Sep 2011
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