Expression analysis of selected haemocyte transcripts from black tiger shrimp infected with yellow head virus

Lerdchai Chintapitaksakul^a,e, Apinunt Udomkit^a, Duncan R. Smith^a, Sakol Panyim^a,b, Burachai Sonthayanon^a,c,d,*

ABSTRACT: Yellow head virus (YHV) is a lethal Penaeid shrimp virus that has caused significant losses to aquaculture farms raising black tiger shrimp (Penaeus monodon). In order to probe some of the underlying molecular events occurring in P. monodon cells in response to YHV infection, we investigated the response of 96 selected shrimp haemocyte transcripts from 90 distinct genes at 24 hours post infection using cDNA macroarray hybridization. We found that the transcripts encoding for PMCP423 (innexin-2-like protein) and NF229 (optomotor blind-like protein) showed increased expression, while those encoding for PMC252 (clathrin light chain) and PMC238 (selenoprotein W-like) showed a decreased expression. Interestingly, no significant alteration in expression levels was noted for several transcripts involved in immune or defence functions such as the putative anti-lipopolysaccharide factor, proteinase inhibitor, or crustin-like antibacterial protein. The reduced level of expressed selenoprotein W transcript in YHV-infected shrimp haemocytes might be related to systemic apoptosis and death of the shrimp.

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^a	Institute of Molecular Biology and Genetics, Mahidol University, Salaya campus, Nakhon Pathom 73170,Thailand
^b	Department of Biochemistry, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400,Thailand
^c	Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
^d	National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Ministry of Science and Technology, Thailand Science Park, 113 Paholyothin Road, Amphoe Khlong Luang, Pathum Thani 12120, Thailand
^e	Bureau of Biotechnology for Animal Production, Department of Livestock Development, Ministry of Agriculture and Cooperatives, Pathum Thani 12000, Thailand

* Corresponding author, E-mail: burachai.son@biotec.or.th

Received 18 Oct 2007, Accepted 12 Mar 2008

Expression analysis of selected haemocyte transcripts from black tiger shrimp infected with yellow head virus

Lerdchai Chintapitaksakula,e, Apinunt Udomkita, Duncan R. Smitha, Sakol Panyima,b, Burachai Sonthayanona,c,d,*

Lerdchai Chintapitaksakul^a,e, Apinunt Udomkit^a, Duncan R. Smith^a, Sakol Panyim^a,b, Burachai Sonthayanon^a,c,d,*