Research articles
ScienceAsia 36 (2010): 26-32 |doi:
10.2306/scienceasia1513-1874.2010.36.026
Effect of culture medium and genotype on germination of hybrid oil palm zygotic embryos
Supawadee Thawaro*, Sompong Te-chato
ABSTRACT: Nutritional requirements for germination of mature zygotic embryos of four genotypes of Elaeis guineensis Jacq. Dura × Pisifera were assessed. A factorial experiment was conducted with four genotypes from separate crossing events and three culture media to show Interactions between genotypes and culture media on seedling germination (p<0.05). The highest frequency of normal seedling germination was facilitated by 1/2 Murashige and Skoog (MS) medium (29.2%), followed by MS (15.0%) and Blaydes (12.9%). Speed of shoot formation followed a similar trend. Roots were induced most rapidly in 1/2 MS medium, followed by Blaydes and then MS. Root induction was similar in 1/2 MS medium (2.29%) and Blaydes medium (1.25%) but only significantly higher than in MS (0.00%).
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Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand |
* Corresponding author, E-mail: stechato@yahoo.com
Received 7 May 2008, Accepted 12 Jan 2010
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