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Volume 48 Number 5
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Volume 48 Number 4 Volume 48 Number 5

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Research articles

ScienceAsia 48 (2022): 635-641 |doi: 10.2306/scienceasia1513-1874.2022.087


Characterization, genome annotation, and antibacterial properties of Actinopolyspora saharensis BKK2


Sirilak Namwonga,*, Suchanat Pandeya, Masahiro Yukib, Takuji Kudob, Moriya Ohkumab, Somboon Tanasupawatc

 
ABSTRACT:     Strain BKK2 was highly similar to Actinopolyspora saharensis (A. saharensis) DSM 45459T based on phenotypic characteristics, chemotaxonomic properties, and genotypic features. It had NaCl ranges of 10?30% (w/v), light-yellow diffusion pigment, Type IV cell wall, Type A whole-cell sugar pattern, Type PIII phospholipid pattern, and no mycolic acid. Moreover, the genotypic features of the strain BKK2 were 98.56% average nucleotide identityBlast (ANIb), 99.03% average nucleotide identity-MUMmer (ANIm), and 90.90% digital DNA-DNA hybridization (dDDH). It was, therefore, identified as A. saharensis. Gene clusters of biosynthetic pathways of this strain are predicted to synthesize desferrioxamine E by complete biosynthesis genes (IucA/IucC and DesABC). A. saharensis BKK2 displayed antibacterial activity against E. coli ATCC 25922 and S. aureus ATCC 25923 at 1.5?2.0 folds weaker than the reference drugs. The results suggested that A. saharensis BKK2 produced at least one bioactive compound likely to be therapeutically useful in the future.

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a Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300 Thailand
b Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074 Japan
c Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand

* Corresponding author, E-mail: sirilak.na@ssru.ac.th

Received 19 Nov 2021, Accepted 3 Apr 2022