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Volume 47 Number 6 Volume 48 Number 1

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Research articles

ScienceAsia 48 (2022): 15-22 |doi: 10.2306/scienceasia1513-1874.2022.004

Efficacy of a standardized Centella asiatica (ECa 233) extract against allodynia in a mouse model of temporomandibular osteoarthritis

Nattapon Rotpenpiana,b, Aree Wanasuntronwongc, Sompol Tapechuma, Chit Carea, Atitaya Roumwongd, Kanokwan Tilokskulchaia, Mayuree H. Tantisirae, Narawut Pakaprota,*

ABSTRACT:     Prolonged inflammation causing tissue injury can induce chronic pain hypersensitivity (allodynia and hyperalgesia). Osteoarthritis of the temporomandibular joint (TMJ-OA) is a common cause of chronic allodynia encountered in dental practice, but many currently available treatments induce intolerable side effects. In this study, we investigated the potential efficacy of a standardized Centella asiatica extract (ECa 233) on allodynia in a TMJ-OA mouse model established by an injection of complete Freundís adjuvant (CFA) into the TMJ. After CFA injection, animals daily received oral administration of vehicle, 0.14 g/kg ibuprofen (positive control), 30 mg/kg ECa 233, or 100 mg/kg ECa 233. Behavioral pain responses were examined by air-puff tests before and after CFA injection on days 3, 7, 14, 21, and 28. On day 28, TMJ-OA pathology was assessed by changes in articular cartilage thickness and graded according to the Osteoarthritis Cartilage Histopathology Assessment (OCHA) system. In the CFA + vehicle group, pain response scores increased gradually, reaching statistical significance compared to untreated Sham controls on days 14, 21, and 28. On day 28, OCHA grade score was 3.5 ± 0.35, and articular cartilage thickness was reduced compared to the Sham group. Both ECa 233 doses significantly attenuated pain response scores and also slowed degeneration of the TMJ with efficacy comparable to ibuprofen. We conclude that ECa 233 can protect against mechanical allodynia and cartilage degeneration in a mouse model of TMJ-OA.

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a Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 Thailand
b Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Songkhla 90110 Thailand
c Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400 Thailand
d Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
e Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131 Thailand

* Corresponding author, E-mail: narawut.pak@mahidol.ac.th

Received 4 May 2021, Accepted 19 Jun 2021