ScienceAsia 48 (2022): 159-164 |doi:
Prevalence and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus clones: A study at Taksin
Hospital, Bangkok, Thailand
Chanwit Tribuddharata, Chalermsri Pummangurab, Maytinee Srifuengfunga, Piyawan Pipobwatthanac, Huttaya Thuncharoonc, Vipavee Rodjunb, Piriyaporn Chongtrakoola, Somporn Srifuengfungb,*
ABSTRACT: : Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in human diseases. Thirtyseven clinical clones from different patients were tested for a molecular study of the mecA gene and multilocus
sequence types (ST). Total genomic extraction, followed by a polymerase chain reaction (PCR) for DNA amplification
with specific primers for mecA, and specific primers for various ST were used. Molecular typing for the study of
genetic relationships among clones was performed by enterobacterial repetitive intergenic consensus (ERIC)-PCR.
Antimicrobial susceptibility testing for all clones to 9 drugs was performed by the disk diffusion and vancomycin Etest. The presence of mecA was detected in all clones. The most common ST was MRSA-ST30, accounting for 81.1% of
all MRSA tested, followed by MRSA-ST8/ST97/ST779 (8.1%), MRSA-ST239 (2.7%) and MRSA-nontypeable clones
(8.1%). Molecular typing by ERIC-PCR demonstrated DNA fingerprints with corresponding results with sequence
types. All clones were susceptible (70?100%) to fosfomycin, fusidic acid, gentamicin, tetracycline, trimethoprimsulfamethoxazole and vancomycin [minimal inhibitory concentration (MIC) range, MIC50 and MIC90 were 0.25?
1.0, 0.5 and 0.75 ?g/ml, respectively by using E-test] but resistant to ciprofloxacin, clindamycin and erythromycin.
Inducible macrolide, lincosamide-type B streptogramin resistance (iMLSB) phenotype was 5.4% while constitutive
MLSB phenotype was 91.9%. For MRSA-ST30 clones, 96.7% were multi-drug resistant (MDR) with the most common
pattern being resistant to ciprofloxacin, clindamycin and erythromycin. These results suggest the importance of MRSA
in the field of epidemiology at a hospital in Thailand.
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||Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 Thailand
||Faculty of Pharmacy, Siam University, Bangkok 10160 Thailand
||Microbiology Laboratory, Taksin Hospital, Bangkok 10600 Thailand
* Corresponding author, E-mail: firstname.lastname@example.org
Received 16 Apr 2021, Accepted 13 Nov 2021