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Volume 46 Number 4 Volume 46 Number 5

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Research articles

ScienceAsia 46 (2020): 530-538 |doi: 10.2306/scienceasia1513-1874.2020.069

Molecular basis of cross-resistance in Sagittaria trifolia L. against acetolactate-synthase inhibitors

Danni Fua,c, Bochui Zhaob,c, Xiuwei Lic, Songhong Weic, Mingshan Jic,*

ABSTRACT:     Herbicide resistance to sulfonylureas in Sagittaria trifolia L. is a common problem in northern China. In our study, we used whole-plant dose-response, acetolactate synthase (ALS) sequencing, and ALS enzyme activity methods to assess 3 putative resistant (LN-1, LN-2, and LN-3) and one susceptible (S) S. trifolia populations for cross-resistance to ALS inhibitors (bensulfuron-methyl, ethoxysulfuron, pyrazosulfuron-ethyl, penoxsulam, pyribenzoxim, and bispyribac-sodium). Regarding the whole-plant dose-response and the in vitro ALS assays, the results showed that the LN-2 population evolved cross-resistance to all herbicides tested whereas the other two populations showed cross-resistance only to bensulfuron-methyl, ethoxysulfuron, pyrazosulfuron-ethyl, and penoxsulam. Results of the in vitro ALS assays were consistent with whole-plant dose-response data. The DNA sequencing of the ALS gene showed that LN-1 and LN-3 populations had a single nucleotide polymorphism in the Pro 197 codon, resulting in the substitution of proline (Pro) by serine (Ser) and leucine (Leu), respectively. However, the LN-2 population showed single nucleotide polymorphism in the second position of the Asp 376 codon, causing substitution of aspartic acid (Asp) by glutamic acid (Glu). The cross-resistance of the S. trifolia population to all ALS inhibitors due to Asp 376 mutation in the ALS gene of this species is reported for the first time.

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a Inner Mongolia University for Nationalities, Tongliao 028000 China
b Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035 China
c College of Plant Protection, Shenyang Agricultural University, Shenyang 110866 China

* Corresponding author, E-mail: biops2016@163.com

Received 13 Aug 2019, Accepted 11 Jul 2020