ScienceAsia 45 (2019): 555-561 |doi:
Characterization and in vitro properties of
Lactobacillus plantarum and Leuconostoc mesenteroides
for probiotic potential and nitrite degradation
Jiang Jikanga, Li Wenxiangb,*
ABSTRACT: In the present work, the nitrite degradation activity and probiotic potential of heterofermentative
Leuconostoc mesenteroides (LM) and homofermentative Lactobacillus plantarum (LP) were demonstrated. The potential
probiotic properties of these bacteria were determined in vitro based on their antimicrobial activity, antibiotic resistance,
aggregation properties, hydrophobicity, survival under simulated gastrointestinal tract conditions, and hemolytic
activity. The results suggested that these two lactic acid bacteria (LAB) species possess hydrophobicity as they exhibited
microbial adhesion to xylene, chloroform, and ethyl acetate. The two strains also showed general resistance to
simulated gastric and intestinal conditions. The LM was resistant to erythromycin, chloramphenicol, norfloxacin, and
vancomycin, while the LP showed resistance to clindamycin, tetracycline, erythromycin, norfloxacin, and vancomycin.
Both LAB were efficient antimicrobials toward Escherichia coli and Staphylococcus aureus based on their inhibition zones.
Furthermore, these LAB showed good tolerance toward nitrite, and displayed α- and γ-hemolysis. These results suggest
that LM and LP are promising probiotic candidates.
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||University of Science and Technology of China, Hefei, Anhui 230026 China
||CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology,
Chinese Academy of Sciences, Suzhou, Jiangsu 215163 China
||Qingdao Agricultural University, Qingdao, China
||Shandong Provincial Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, China
* Corresponding author, E-mail: email@example.com
Received 25 Jul 2019, Accepted 23 Nov 2019