ScienceAsia 32 (2006): 429-435 |doi: 10.2306/scienceasia1513-1874.2006.32.429

Validated Spectrophotometric Method for the Assay of Nifedipine in Bulk and Commercial Dosage Forms

Nafisur Rahman^* and Syed Najmul Hejaz Azmi

 
ABSTRACT:A validated spectrophotometric method has been described for the assay of nifedipine in bulk and commercial dosage forms. The method is based on the oxidation of drug with Fe(III) at pH 1.42. The reduced Fe(II) subsequently reacts with 1,10-phenanthroline to produce a red colored tris(1,10-phenanthroline) iron(II) complex, which absorbs maximally at 505 nm. Beer’s law is obeyed in the concentration range 0.5 - 14.0 µg mL-1 with molar absorptivity and Sandell’s sensitivity of 2.805×104 L mol-1 cm-1 and 0.012 µg cm- 2 per 0.001 absorbance unit, respectively. The various experimental parameters were optimized. The method has been successfully applied for the quantitation of the drug in commercial dosage forms. The results of analysis were statistically compared with those of a reference method showing acceptable recovery and precision. The experimental true bias of all samples based on recovery experiments were within ± 2 %.

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Department of Chemistry, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India.

* Corresponding author, E-mail: cht17nr@yahoo.co.in

Received 5 Apr 2006, Accepted 5 Jun 2006